Skip to main content
U.S. Edition
Sunday, February 28, 2021

Reverse transcription polymerase chain reaction

Laboratory technique to multiply an RNA sample for study


Reverse transcription polymerase chain reaction
Reverse transcription polymerase chain reaction

Reverse transcription polymerase chain reaction (RT-PCR) is a laboratory technique combining reverse transcription of RNA into DNA and amplification of specific DNA targets using polymerase chain reaction (PCR). It is primarily used to measure the amount of a specific RNA. This is achieved by monitoring the amplification reaction using fluorescence, a technique called real-time PCR or quantitative PCR (qPCR). Combined RT-PCR and qPCR are routinely used for analysis of gene expression and quantification of viral RNA in research and clinical settings.

0 shares 1 views

News coverage

You might like

Reverse transcription polymerase chain reaction media coverage